4.0 Identification of Secondary Structure

4.2 Identification without 3D Structure

In the continuing search for relationships between the structure and function of biological macromolecules, the study of conformation (preferably in a native-like environment) must play an important role. The problem of determining the three-dimensional structure of a macromolecule is a formidable one and no single technique is completely adequate. Today, both X-ray and NMR techniques are capable of producing structures at atomic resolution. However, these methods are quite laborious and each is beset with its own unique set of limitations (and advantages). Fortunately, many interesting can be explored and answered without knowledge of the exact three-dimensional structure. A number of experimental techniques can selectively examine certain general aspects of macromolecular structure while completely ignoring others - usually with relatively little investment of time and sample. Among those techniques more commonly used are "Circular dichroism spectroscopy" , "NMR spectroscopy" and "Infra-red" spectroscopy.

4.2.1 - Circular dichroism spectroscopy
4.2.2 - NMR spectroscopy
4.2.3 - FT-infrared spectroscopy

No Title - 23 JUL 96

written by Kurt D. Berndt

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